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BEGINNERS SAFETY MANUAL FOR
OLYMPUS FV3000 CONFOCAL (#02-20)
Produced by Shaalini, Graham Wright, Fiona Chia, Cris Barzaghi, Melvin
1. Safety Guidelines ................................................................................................................... 2
1.1 Lasers ............................................................................................................................... 2
1.2 Immersion Oil ................................................................................................................... 2
1.3 Metal Halide Lamps (MHL) .............................................................................................. 2
2. Training .................................................................................................................................. 3
2.1 To arrange a training session: .......................................................................................... 3
3. Online Booking and System Access........................................................................................ 4
3.1 Acknowledgements .......................................................................................................... 5
4. Operation Procedures ............................................................................................................ 5
4.1 Switching ON Protocol ..................................................................................................... 5
4.2 Objective lens ................................................................................................................... 6
4.2 Software Initiation ........................................................................................................... 7
4.3 Setting up directory to save images ................................................................................ 8
4.4 Laser Configuration Settings ............................................................................................ 9
4.5 Using the LSM Imaging Tab ............................................................................................ 10
4.6 Image Adjustments - Setting the Gain and Offset properly.......................................... 11
4.7 How to do a Z stack for a 3D Image (xyz) ....................................................................... 11
4.8 For Time-lapse (xyzt) ...................................................................................................... 12
4.9 Reuse previous settings and export images .................................................................. 12
4.10 Switching OFF Protocol ................................................................................................ 13
5. Troubleshooting ................................................................................................................... 14
Last updated on Mar 2022