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1st offence

A warning will be issued along with the reminder of the rules.

2nd offence

A second warning will be issued and your respective PI will be notified.

3rd offence

Banned from using any of the facility's microscope for 2 weeks.

3.1 Acknowledgements

If you use the TLL Microscopy and Imaging facility and/or have been trained or assisted any

of the bioimaging facility members in your research, then this should be acknowledged

appropriately in your publications and presentations.

Every confocal system in TLL Bioimaging Facility has its specific instructional manual which

are found in every confocal rooms. Strictly adhere to the correct order of operation for all

system. Failure to do so will result in disciplinary action from the facility. Any issues

encountered during the operation of the system are advised to seek help from the

Bioimaging facility.

Modification, exchange or removal of components beyond this operational manual is strictly

prohibited and is only carried out by the manufacturer, Bioimaging facility or by experienced

users approved by bioimaging facility. During operation of laser microscopy system, do not

look into the laser beam directly as they are all Class 3b and Class 4 lasers.

(a) SIGN IN the Logbook and record your START-TIMING.

(b) Switch on the Master switch on the wall (1) & PC (2). Wait until windows icon pops

up.

(d) (Optional) Turn on the Metal Halide lamp (5), if needed.

(e) Turn on the Touch screen panel power button at the back (6) and wait for 5s to light

up.

(f) Switching ON Lasers,

• To use 405/488/561/640nm, switch on the power button on the main

combiner (7), then turn the key to "ON" (7A) and finally turn on the

individual lasers that you need.

• To use 445/514nm, switch on the power button on the sub combiner (8),

then turn the key to "ON" (8A) and finally turn on the individual lasers that

you need.

(g) (Optional) Turn on the incubator (9) if required. It takes 10-15mins to stabilize.

(h) Mount your slide onto the sample holder. If you need Live cell Imaging, turn on the

OkaLab incubator and the facility CO

help mounting the stage incubator holder onto the stage, please request help from

the TLL bioimaging department.

(i) Log in with your TLL user account and double click on "FV31S-SW" to load the

software.

(10). It takes 10mins to stabilize. If you require

Last updated on Mar 2022