Olympus IX2-RFSPOT Petunjuk

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The IX2-RFSPOT has been designed to be used in place of the field iris diaphragm (FS) of a IX2 Series reflected

fluorescence illuminator (IX2-RFAL). When the IX2-RFSPOT is used, a desired pinhole* can be projected on the

specimen plane, which is convenient for canceling the cage on caged specimens.

* No pinhole is provided with the unit. Please fabricate a pinhole yourself (see the figure illustrated later in this

manual) or use the commercially available recommended product (pinhole manufactured by Melles Griot, outer

diameter 16 mm).

External View

Clamping ring

Washers

(x 4)

Microscope frame side

Focus adjustment screw

Precautions for Operation

· Flare is produced around the pinhole. Ghost may be noticeable when the pinhole position is deviated from the center of

the field of view.

· The pinhole image may not be able to be focused perfectly depending on the objective and wavelength in use.

· Centering of the pinhole guarantees only the movement in the center of the field of view. Movement to the peripheral

section of the field is not possible.

· The pinhole position may be deviated slightly when the pinhole is engaged or disengaged. Perform centering whenever

strict positioning is required.

· The pinhole cannot be set to an intermediate position between the IN and OUT positions.

· The IX2-SFR stage gets in the way of centering the pinhole. To prevent it, remount the IX2-SFR with the front in the back or

replace it with the IX-SVL-2.

PINHOLE UNIT

IX2-RFSPOT

Lamp housing side

Centering screws

Pinhole engagement knob

Pushed in: Pinhole engaged (IN)

Pulled out: Vacant hole (OUT)

Clamping screw

INSTRUCTIONS

A X 7 3 8 3

IX2-RFSPOT