Olympus IX2-RFSPOT Petunjuk - Halaman 2

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Installation

CAUTION: To ensure safety, be sure to turn off the reflected light source before attaching.

Removing the Field Iris Diaphragm

Using the Allen screwdriver provided with the microscope frame, loosen the field iris diaphragm clamping screw on the

IX2-RFAL and pull out the field iris diaphragm to remove it.

Attaching the Pinhole

1. Using the clamping ring mounting tool provided with the pinhole unit, turn the clamping ring counterclockwise to remove.

2. Drop in the specified number of washers, determined according to the objective and excitation wavelength to be used

(see the list shown later in this manual), in the position where the clamping ring has been placed.

}If the number of washers is wrong, it will not be possible to focus on the pinhole.

3. Place the pinhole, fabricated by you or purchased commercially, above the washers, then clamp by attaching the

clamping ring again.

For the orientation of the pinhole, follow the instructions given with the figure if the pinhole is fabricated by you or insert

with the indications facing down (the lamp housing side) if the pinhole is a commercially available product.

Attaching the Pinhole Unit

Insert the pinhole unit in the microscope position where the field iris diaphragm has been, and clamp the pinhole unit

using the field iris diaphragm clamping screw.

Operation

}Perform centering in the same way as the field iris diaphragm centering method described in the instruction manual for

the reflected fluorescence system, so that the pinhole is visible in the center of the field of view.

Focusing the Pinhole

Fit the Allen screwdriver into the focus adjustment screw and turn to adjust. (The adjustment range is ±1 mm.)

Centering the Pinhole

Insert the Allen screwdriver alternately into the two centering screws and rotate them so that the pinhole comes at the

center of the field of view.

Engaging/Disengaging the Pinhole

1. Pull out the engagement knob completely to disengage the pinhole from the light path.

2. To re-engage the pinhole in the light path, gently push the engagement knob till the deep.