Olympus ST. HS Petunjuk Manual - Halaman 7

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V How to Use the Microscope

1. IUumination

What is most important in a microscopic work is illumination, because the light source and condenser greatly

influence the resolving power of the microscope lenses.

For microscope illumination, natural daylight or artificial

light

source is used. Normally it is sufficient to use

natural light coming through a window. However, in some cases such as photomicrography, a specially designed

microscope illuminator is used.

When daylight is used

Avoid the direct rays of the sun.

is most desirable that the microscope is placed

a window facing north.

Usually the plane mirror is used but the concave side is used when annoying renections of the window frame or

trees come into the field of view.

When an illuminator is used

A low-voltage bulb is used for microscope illumination.

Olympus puts on the market a specially designed micro-

scope illuminator LSD (u!:ing a 6V, SA transformer,

optical accessory). When this illumination apparatus is

used, the beam of light must

adjusted by adjusting the

position of the bulb and the plane side of the mirror should

used. The accessory daylight mter (cobalt color)

shoHld be inserted into the filter holder.

Centering

When a microscope illuminator is used, adjust it so that the axis of the illuminating system falls in the center 01

the mirror. Next, rack the body tube up and down SO that it may

roughly in a position in which the

microscope is used for observing the specimen.

Remove the eyepiece and look through the eyepiece sleeve, and you will see the back lens of the objective.

Adjust the mirror SO that the back lens is evenly illuminated.

Similar procedures must be adopted when daylight is used.

Slide glass and cover glass

Ow microscopes are designed to use the slide glass and cover glass of the following thicknesses.

Slide glass:

0.8 - 1.0mm

Cover glass:

0.16 - 0.18mm

Use slide and cover glasses that satisfy these requirements.

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