ページを印刷するV How to Use the Microscope
1. IUumination
What is most important in a microscopic work is illumination, because the light source and condenser greatly
influence the resolving power of the microscope lenses.
For microscope illumination, natural daylight or artificial
light
source is used. Normally it is sufficient to use
natural light coming through a window. However, in some cases such as photomicrography, a specially designed
microscope illuminator is used.
When daylight is used
Avoid the direct rays of the sun.
It
is most desirable that the microscope is placed
by
a window facing north.
Usually the plane mirror is used but the concave side is used when annoying renections of the window frame or
trees come into the field of view.
When an illuminator is used
A low-voltage bulb is used for microscope illumination.
Olympus puts on the market a specially designed micro-
scope illuminator LSD (u!:ing a 6V, SA transformer,
optical accessory). When this illumination apparatus is
used, the beam of light must
be
adjusted by adjusting the
position of the bulb and the plane side of the mirror should
be
used. The accessory daylight mter (cobalt color)
shoHld be inserted into the filter holder.
Centering
When a microscope illuminator is used, adjust it so that the axis of the illuminating system falls in the center 01
the mirror. Next, rack the body tube up and down SO that it may
be
roughly in a position in which the
microscope is used for observing the specimen.
Remove the eyepiece and look through the eyepiece sleeve, and you will see the back lens of the objective.
Adjust the mirror SO that the back lens is evenly illuminated.
Similar procedures must be adopted when daylight is used.
Slide glass and cover glass
Ow microscopes are designed to use the slide glass and cover glass of the following thicknesses.
Slide glass:
0.8 - 1.0mm
Cover glass:
0.16 - 0.18mm
Use slide and cover glasses that satisfy these requirements.
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