Olympus U-UCD8A Petunjuk Manual - Halaman 12

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3-3 Phase Contrast Observation (PH)

Applicable objective power

Power

Phase Contrast (PH)

* With the super-widefield observation (FN 26.5), flare may be observed in the peripheral areas of the field of

view. However, this does not pose problem in photomicrography.

}When a transmitted light DIC slider (U-DICT, U-DICTS, etc.) is used in combination, pull out the slider until

it clicks in place to disengage the slider from the light path.

}When a reflected light analyzer (U-AN) is used in combination, pull out the analyzer until it clicks in place

to disengage the analyzer from the light path.

1. Select the turret containing the phase contrast ring (U-PH1S, U-PH2S, U-PH3S or U-PH3) matching the

objective in use.

2. Mount the phase contrast objective to be used in the revolving nosepiece and rotate the nosepiece to

engage the objective in the light path.

3. Open the aperture iris diaphragm.

# When the aperture iris diaphragm is stopped down, flare may occur at the center.

4. Place the specimen on the stage and move the stage up or down to bring the specimen in focus.

5. Remove the eyepiece from the eyepiece sleeve and replace with the U-CT30 centering telescope.

10X

20X

40X

¦*

Top lens IN

60X

100X