Olympus BX51 Manuale operativo - Pagina 7

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1. Turn the halogen bulb switch (1) for reflected light to "│" (ON). Engage the bright field (BF) filter (17).

2. Adjust the light path.

‐ The light path selector knob (2) should be pushed in all the way.

‐ The knob on the reflected light splitter (30) should be pushed in all the way.

‐ The shutter knob (20) should be slid to the position marked "○"

3. Select reflected light (upper position) on the switch by the brightness indicator (4).

4. Adjust the light intensity using the brightness adjustment knob (3). The light intensity scale (4) indicates the

lamp voltage. Deselect the "PRESET" button (not illuminated green) to avoid maximum illumination.

5. Adjust the interpupillary distance. While looking through the eyepieces, adjust the eyepieces (5) until the

left and right fields of view coincide.

6. Swing the 2.5x objective (6) in place.

7. Place the specimen on the stage (7).

8. Find the specimen using the stage controls (9).

9. Focus on the specimen using fine/course focusing knobs (10).

10. Adjust the diopter:

‐ Close your left eye and focus on the specimen using the fine focus knob.

‐ Close your right eye and focus on the specimen using the diopter ring (11) on the left eyepiece.

‐ Open both eyes and confirm that it is in focus.

11. Switch to the next objective by rotating the nosepiece (12) and focus. Continue until you reach the desired

magnification.

12. Establish Koehler illumination:

‐ Close the field iris diaphragm (13) until you can see the edges.

‐ Focus the image of the field iris diaphragm by raising or lowering the condenser using the condenser

height adjustment knob (14).

‐ Check whether the light is centered in the field of view. If not, use the condenser centering screws

(15) to move the field iris diaphragm image to the center of the field of view.

‐ Open the field iris diaphragm until its image circumscribes the field of view.

‐ Match the opening of the condenser aperture iris diaphragm (16) with the N.A. of the objective in

use to achieve the optimum objective performance.

13. Examine specimen and take a picture, if needed. (See page 9 for details).

14. When finished:

‐ Lower the stage by turning the focus knob (10) and remove the specimen.

‐ Turn the nosepiece (12) until the 2.5x objective is into place.

‐ Lower the light intensity to zero using the brightness adjustment knob (3)

‐ Turn the halogen light switch (1) to "

‐ Return any slide filters (26) (31) (32) to their disengaged positions.

BRIGHT FIELD Reflected light

White light is shone onto a reflective sample from above.

○

" (OFF).

(OPEN).