Olympus BX51 Manuale operativo
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BX51 Microscope
Bright field halogen lamp ; reflected (top) or transmitted (bottom) illumination
Fluorescence mercury lamp
Filter positions
Objective
Magnification Immersion N.A. Working Distance Filters
M Plan Fluorite
UM Plan Fluorite
UM Plan Fluorite
LM Plan Fluorite
LM Plan Fluorite
LM Plan Fluorite
Filters
Wheel Position 1 – HQ:Rdil red
Position 2 – DAPI, fluorescence filter for fluorophores, nuclear stain
Position 3 – DIC Nomarski Differential Contrast filter
Position 4 – BF, bright field (for all white light)
Position 5 – DF, dark field filter
Position 6 – Qdot hxCM red/blue
Cooke SensiCAM Camera
Black and White camera
ImagePro Plus Software
Capture single images (8‐bit or 12‐bit) or movies (.avi files)
Image adjustments (gamma, saturation, background subtraction, etc.)
Measure distances
Add labels or scale bars
Color adjustments and false color
Olympus BX51 Microscope
1. HQ:RDil (red)
2. DAPI (340‐380 Ex; 400 dichroic; 435‐485 Em)
3. DIC
4. bright field
5. dark field
6. Qdot hxCM (red/blue)
2.5X
Dry
5X
Dry
10X
Dry
20X
Dry
50X
Dry
100X
Dry
(May 2014)
0.08
0.15
0.30
0.40
0.50
0.80
10.7
bright field only (w/polarizer)
12.0
all
11.0
all
12.0
all
10.6
all
3.4
all (available upon request)
1