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BX51 Microscope

Bright field halogen lamp ; reflected (top) or transmitted (bottom) illumination

Fluorescence mercury lamp

Filter positions

Objective

Magnification Immersion N.A. Working Distance Filters

M Plan Fluorite

UM Plan Fluorite

LM Plan Fluorite

Filters

Wheel Position 1 – HQ:Rdil red

Position 2 – DAPI, fluorescence filter for fluorophores, nuclear stain

Position 3 – DIC Nomarski Differential Contrast filter

Position 4 – BF, bright field (for all white light)

Position 5 – DF, dark field filter

Position 6 – Qdot hxCM red/blue

Cooke SensiCAM Camera

Black and White camera

ImagePro Plus Software

Capture single images (8‐bit or 12‐bit) or movies (.avi files)

Image adjustments (gamma, saturation, background subtraction, etc.)

Measure distances

Add labels or scale bars

Color adjustments and false color

Olympus BX51 Microscope

1. HQ:RDil (red)

2. DAPI (340‐380 Ex; 400 dichroic; 435‐485 Em)

3. DIC

4. bright field

5. dark field

6. Qdot hxCM (red/blue)

2.5X

Dry

10X

Dry

20X

Dry

50X

Dry

100X

Dry

(May 2014)

0.08

0.15

0.30

0.40

0.50

0.80

10.7

bright field only (w/polarizer)

12.0

all

11.0

all

12.0

all

10.6

all

3.4

all (available upon request)