Olympus Fluoview-1000 Manuale d'uso - Pagina 18

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Olympus Fluoview-1000 User's Guide

V.M. Bloedel Hearing Research Center, Core for Communication Research

Center on Human Development and Disability, Digital Microscopy Center

2.6.6 Box Size effect on resolution

The resolution of an image is determined by dividing the scanned field by the box size to set the area

represented by each pixel in the final image. Dividing the field into fewer sample points requires that

each sample point will encompass a larger area. Conversely, doubling the box size (e.g. from 512X 512

to 1024X1024) reduces the area of each sample point by 2-fold. However, a 2-fold increase in the box

size will also give a 4-fold increase of the file size because this is an area (width X height).

The box size and zoom setting (section 2.10) are used together to control the size and resolution of the

scanned region for any selected lens.

Refer to the later section on Optimizing the Image.

This only needs to be set once after a user account is established. If you don't activate the Auto HV

setting, then every time laser and HV are set with a selected dwell time (such as with Scan X2 or Scan

X4), switching to a different dwell time may cause your image to become saturated.

1. Begin scanning with Focus X2 or Focus X4 Focus mode and find your sample;

2. Adjust the laser and detector settings;

3. Click on 'Auto HV' to set the reference histogram;

4. Switch to Repeat XY or XY to scan with a longer dwell time, e.g. 4 µs;

5. Notice that the HV is reduced to maintain image intensity and prevent saturation.

2.7

This panel controls the scanned area.

The circle represents the area imaged by the objective lens. The scanner will record the selected sub-

region.

This is the portion of the objective field of view that will be recorded. A square region at Zoom = 1

represents the maximum field, as displayed in figure 8. A Zoom = 2 would scan only the central 1/2 of

the area.

The scan region may be freely rotated by clicking on the red dot and dragging it around the circle.

Clicking on the curved arrows will increment rotation in 0.1° increments.

May 11, 2011