Olympus Fluoview-1000 Manuale d'uso

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1. Add a Scale Bar to Your File

V.M. Bloedel Hearing Research Center, Core for Communication Research

Center on Human Development and Disability, Digital Microscopy Center

Quick Start Guide

Start-Up

Shut-Down

Quick Guide to Getting an Image

Place the sample on the microscope

Finding the sample - transmitted light or DIC

Brightfield vs. DIC

Finding the sample - epi-fluorescence

Finding the sample - confocal

Setting Up for Confocal Microscopy

Choosing the Dye

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Changing the Dye Selection

Confocal acquisition controls.

Settings for the fluorescence detectors.

Additional detector controls.

Laser Controls.

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Line Selection.

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Line availability, Figure 7.2.

Laser Power Selector, Figure 7.3.

Scroll List, Figure 7.4.

Line Settings, Figure 7.5

Scan Controls

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Scan Mode, Figure 6.1

Dwell Time, Figure 6.2

Scan Regions, Figure 6.3.

Image Aspect Ratio, Figure 6.4.

Box Size, 6.5.

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Box Size effect on resolution

AutoHV, 6.6.

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Scan Area Controls

Field of View, 8.1.

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Scan Area, 8.2.

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Scan Rotation, 8.3.

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Scan Zoom, 8.4.

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Reset Scan Values, 8.5.

Scan Offset, 8.6.

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Use of Sequential, Simultaneous and Averaging.

Simultaneous Capture versus Sequential Capture.

Two Methods for Sequential Capture

Sequential Line Capture, Figure 9.1

Sequential Frame Capture, Figure 9.1

May 11, 2011

Olympus Fluoview-1000 User's Guide

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