Olympus Fluoview-1000 Manuale d'uso - Pagina 3

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1. Add a Scale Bar to Your File

V.M. Bloedel Hearing Research Center, Core for Communication Research

Center on Human Development and Disability, Digital Microscopy Center

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Reducing bleedthrough

Procedure to minimize bleedthrough

Noise.

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Signal Noise (Shot Noise).

Detector Noise.

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Kalman Averaging.

Background

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Bright Images versus Scorching the Specimen

Images with Weak Signals

Using Zoom

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Zoom reduces objective lens aberrations.

Use Zoom to fulfill sampling requirements for the specimen.

Use Zoom to fulfill adequately sample the objective.

Obtaining a Z-Series.

Resolution.

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Sampling frequency and the Nyquist Theorem.

Box size.

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Zoom. 38

Considerations for increasing resolution.

Size of Pixels

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Table. 5 Pixel Size (µm) versus Box Size and Magnification at Zoom = 1

Microscope controls.

Focus.

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Fine/Coarse Focus.

Lowering the Objectives.

Manual shutter.

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Differential Interference Contrast (DIC).

DIC Analyzer.

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DIC polarizer.

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DIC mirror.

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Setting Koehler Illumination.

Condenser position.

Photoactivation Methods.

Zoom versus Laser Power.

Recording initial recovery event.

Methods to photoactivate/bleach.

Using the Stimulus Window.

Avoid spontaneous photoactivation.

Controlling Computer Display Brightness.

Set 'Darkroom' color mode.

Technical Information

Caring for Objectives

May 11, 2011

Olympus Fluoview-1000 User's Guide

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