Olympus U-UCD8A Manuale di istruzioni - Pagina 19

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Olympus U-UCD8A Manuale di istruzioni

3-5 Darkfield Observation (DF)

Applicable objective power
Power
Darkfield (DF)
* With the super-widefield observation (FN 26.5), flare may be observed in the peripheral areas of the field of view.
However, this does not pose problem in photomicrography.
** Objectives with N.A. of no more than 0.7 can be used. (An objective which is equipped with an iris diaphragm
and which can reduce the N.A. to 0.7 or less can also be used.)
}When only a transmitted light DIC slider is used in combination, pull out the slider until it clicks in place to
disengage the slider from the light path.
}When a reflected light analyzer (U-AN) is used in combination, pull out the analyzer until it clicks in place to
disengage the analyzer from the light path.
1. Select the DFA darkfield observation light path.
2. Mount the objective to be used in the revolving nosepiece and rotate the nosepiece to swing the objective in
place.
3. Open the aperture iris diaphragm.
4. Place the specimen on the stage and move the stage up or down to bring the specimen into focus.
5. Remove an eyepiece from the eyepiece sleeve, and look at the objective pupil. Center the darkfield ring using
the optical element centering knob.
10X
20X
40X
¦*
¦**
¦**
Top lens IN
60X
100X
¦**
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U-UCD8A
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