Accuris SmartDoc 2.0 Manuale di istruzioni - Pagina 7
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Stampa la paginaother blue light transilluminators and allows through the emission wavelengths
from fluorescing nucleic acid stains.
Additional filters are available separately for the SmartDoc and are recommended
for using the SmartDoc enclosure on a UV Transilluminator. These glass band
pass filters block the UV light so the UV bulbs will not be seen in the images, and
they also eliminate background wavelengths from the excitation source.
E5001-590
band pass photo filter, 590nm, for imaging EtBR on UV transilluminator
E5001-535
band pass photo filter, 535nm for imaging green stains on UV transilluminator
Caution: Using the SmartDoc on a UV Transilluminator without an
appropriate filter in place can cause eye or skin exposure to UV
radiation. The SmartDoc filters effectively block harmful UV radiation,
and should always be properly installed when using UV illumination.
7. GEL IMAGING WITH THE SMART DOC
The SmartDoc 2.0 Illumination base produces visible blue light with peak output
of 465nm for excitation of common nucleic acid stains. Compatible stains include
Accuris SmartGlow™ PS (Pre Stain), SmartGlow™ LD (Loading Dye), SYBR® Green I
and II, SYBR® Safe, SYBR® Gold, Gel Star, Gel Green, and Green Glo™.
1. Set the gel preparation platform onto a level lab bench or table.
2. Glean the glass surface with alcohol or glass cleaner and allow to dry.
3. Carefully place a stained electrophoresis gel onto the square glass surface
using care to eliminate bubbles under the gel.
4. Note: The maximum sized gel to fit onto the glass surface is 15x15cm, cut
away any overhanging edges of the gel if required.
5. Place the blue illumination base over the gel preparation platform, taking
care that it nests correctly into place.
6. Place the SmartDoc imaging enclosure over the illumination base.
7. Insert the E5001-ORANGE filter into place on the top platform. The side
with the Accuris logo should be facing up.
8. Place a smartphone face down onto the top platform, and align the camera
lens with the filter.
9. Press the power switch on the illumination base to turn on the blue LEDs.
10. Select the Camera Mode on your Smart Phone, and turn off the flash
setting. When properly positioned, the gel will be seen in the device's
display screen. Focus as required.
11. If required, remove or insert the extender piece to maximize the image size
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