cytiva AKTA start Scheda di manutenzione - Pagina 6
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4. Run for 3 to 5 minutes.
Operation from the Instrument
Display
Perform the following operations to clean the
Sample valve.
Set Run parameters
Set Run parameters
Operation from UNICORN start
Perform the following operations to clean the
Sample valve.
Set Run parameters
Set Run parameters
Clean the column
Cleaning
After a run is completed, clean the column if
required.
Instruction
•
Disconnect the column from the flow path
•
Clean the column off-line according to the
column instructions.
6
ÄKTA start
Manual run
Set Flow path
Run
End Run
UNICORN start
System control
Manual run
Set Flow path
Run
End Run
Storage
If the column is not going to be used for a couple of
days or longer, store the column.
Instruction
•
Fill the column with the storage solution
recommended in the column data sheet.
•
Detach the column from the instrument
and store it according to the column
recommendation.
Other maintenance
procedures
Clean the UV flow cell
Maintenance interval
Clean the UV flow cell once every three months, or
when the signal is unstable or readings appear to be
incorrect.
Cleaning solutions
•
10% surfactant detergent solution (e.g., Decon™
90, Deconex™ 11, or RBS™ 25)
•
DM water
Instruction
1. Disconnect the tubing from the top of the UV
flow cell, and replace the fingertight connector
with a Luer connector (needed for connecting a
syringe).
2. Disconnect the tubing from the bottom of the UV
flow cell, connect a piece of tubing of sufficient
length to the UV flow cell and insert the tubing
into a waste container.
3. Fill a syringe with detergent solution and inject
the detergent solution through the UV flow cell
about five times.
4. Leave the UV flow cell filled with cleaning
solution for 15 minutes.
5. Fill a syringe with DM water and rinse the flow
cell thoroughly.
Clean the Conductivity flow cell
Maintenance interval
Clean the Conductivity flow cell when the
Conductivity Monitor shows a slow response or
when the conductivity measurements are not
comparable to previous results.
ÄKTA start Maintenance Cue Card, 29024043 AF