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計測機器 Cole Parmer 1200 SeriesのPDF ユーザーマニュアルをオンラインで閲覧またはダウンロードできます。Cole Parmer 1200 Series 18 ページ。

Concentration Mode

C is used for determining the concentration of unknown samples.

NOTE: This method should only be used when the relationship between Absorbance

and Concentration is known to be linear. The concentration of the Standard solution

used to calibrate the instrument should be higher than the most concentrated sample.

1. Select the desired wavelength by turning the wavelength control knob.

2. Using the MODE button, select A mode.

3. Insert the cuvette containing the blank solution.

4. Set 0.000A with the ∧ button.

5. Using the MODE button, select C mode.

6. Insert a cuvette containing a standard solution of known concentration in the Sample

Compartment and set the Digital Display window to be the value of the standard by

using the ∧ and ∨ buttons.

7. Press the ENT button.

NOTE: If the reading changes, the factor required is too high (i.e. >1999) to be

displayed. In this case, divide the concentration by 10; re-select the C mode by

successive presses on the MODE button, cycling through the F, T, and A modes, and

follow step 2 above to set the concentration of the standard to the reduced value.

8. With the standard concentration set, determine the concentration values of samples

with unknown concentration by inserting the sample cuvette into the Sample

Compartment and reading the value direct from the Digital Display.

9. To read the value of the multiplier used to convert Abs to Concentration, after

measuring all the samples, change the mode to F and read the multiplier from the

display. Keep a record of this value for future use.

Operational Note: if the MODE is changed to read F or A and the Concentration C

reading is "frozen", re-start at step 1.

This is a special mode for measuring concentration values of unknown samples using a

previously determined factor to convert absorbance readings to concentration.

After setting the wavelength, and setting zero Abs on the blank solution, using

the MODE button, select F mode.

Insert a cuvette containing a sample.

Using the ∧ and ∨ buttons, set the Digital Display window to the desired value

of the multiplier.

Press the ENT button. The spectrophotometer switches to the C mode.

Operational Note: If the Concentration of the sample is too high to be displayed, the

instrument will not switch to C mode when the ENT button is pressed. Dilute the sample

and multiply the concentration reading by the dilution factor to obtain the original sample