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計測機器 Cole Parmer 1200 SeriesのPDF ユーザーマニュアルをオンラインで閲覧またはダウンロードできます。Cole Parmer 1200 Series 18 ページ。

Refer to the 1200 Series Spectrophotometer Software User's Manual Version SS-

1.22 for detail.

Maintenance

1. Turn off and unplug the instrument.

2. Remove the lamp access door at the back of the instrument by removing the four set

screws.

3. Unplug the lamp from the white connector. Insert the new lamp; pushing it in as far

as it will go. Replacement lamp: Item Number: S-1200-505 (6V 10W G4 type)

CAUTION: DO NOT HANDLE THE LAMP WITH BARE FINGERS. USE TISSUE OR

CLOTH WHEN HANDLING LAMP.

4. Turn on the instrument. Set the wavelength at 340 nm, insert a test tube or an empty

cuvette with cuvette holder, and blank the instrument. If the energy is low, adjust the

lamp by "pulling" or "pushing" it so that the light beam is focused on the entrance slit

of the monochromator. Since the lamp socket is pre-aligned, there will be minimum,

if any, adjustment required.

5. Re-install the lamp access door.

Normally the Cole Parmer 1200 Series spectrophotometer retains its wavelength

calibration indefinitely. However if the instrument receives a severe shock or is abused,

use the following methods to check wavelength calibration. Please note that this test

requires the Didymium filter, item number S-2100-116, or the Holmium Oxide filter, item

number S-2100-115.

In the filter method, the didymium filter has two distinct absorbance peaks at 529 nm

and 807 nm. The Holmium filter has a distinct peak at 361 nm. When the instrument is

calibrated properly you will find minimum Transmittance (maximum Absorbance) at the

range ± 2 nm from these peaks. Note that the specific Transmittance values are not

important as you are only looking for the wavelength where the minimum Transmittance

(maximum Absorbance) occurs.

Note: If you calibration filter has a certified peak/valley curve attached, please use the

peaks on the curve to verify the instrument.

Holmium Oxide Filter Method

1. Turn instrument on and allow it to warm up for 15 minutes.

2. Select the A mode.

3. Set the wavelength to 350 nm.

4. Make sure the Cuvette Adapter is empty and place it in the Sample Compartment.

Close the lid.

5. Set zero Absorbance by pressing the ∧ button. Wait a few seconds when the display