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1x TAE 40 mM tris (pH 7.6), 20 mM acetic acid, 1 mM EDTA
•
50 x (1l) dissolve in 750 ml distilled water:
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242 g tris base (FW = 121)
•
57.1 ml glacial acetic acid
•
100 ml 0.5 M EDTA (pH 8.0).
•
Fill to 1 litre with distilled water.
1x TBE 89 mM tris (pH 7.6), 89 mM boric acid, 2 mM EDTA
•
10 x (1l) dissolve in 750 ml distilled water:
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108 g tris base (FW = 121)
•
55 g boric acid (FW = 61.8)
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40 ml 0.5 M EDTA (pH 8.0)
•
Fill to 1 litre with distilled water.
Sample Loading Dye
•
10x sample buffer stock consists of 50% glycerol, 0.25%
bromophenol blue, and 0.25% xylene cyanole FF in 1x TAE
buffer. Only 1–10 ml of the 10x loading dye should be pre-
pared.
Ethidium Bromide Solution
•
Add 10 mg of Ethidium Bromide to 1 ml distilled water.
E3xxx
E3xxx
Solutions
Solutions
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