Consort E3100 Manual - Página 11
Procurar online ou descarregar pdf Manual para Equipamento de laboratório Consort E3100. Consort E3100 16 páginas.
Imprimir página
1x TAE 40 mM tris (pH 7.6), 20 mM acetic acid, 1 mM EDTA
•
50 x (1l) dissolve in 750 ml distilled water:
•
242 g tris base (FW = 121)
•
57.1 ml glacial acetic acid
•
100 ml 0.5 M EDTA (pH 8.0).
•
Fill to 1 litre with distilled water.
1x TBE 89 mM tris (pH 7.6), 89 mM boric acid, 2 mM EDTA
•
10 x (1l) dissolve in 750 ml distilled water:
•
108 g tris base (FW = 121)
•
55 g boric acid (FW = 61.8)
•
40 ml 0.5 M EDTA (pH 8.0)
•
Fill to 1 litre with distilled water.
Sample Loading Dye
•
10x sample buffer stock consists of 50% glycerol, 0.25%
bromophenol blue, and 0.25% xylene cyanole FF in 1x TAE
buffer. Only 1–10 ml of the 10x loading dye should be pre-
pared.
Ethidium Bromide Solution
•
Add 10 mg of Ethidium Bromide to 1 ml distilled water.
E3xxx
E3xxx
Solutions
Solutions
9 9