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Optimisation

The MiniSeal 2 System has been designed to reliably seal plates of different heights and different

plastics, using a variety of films, to give a seal with varying properties. These components will

require different sealing conditions.

The quality and strength of the seal created between the sealing film will vary with different

conditions. In general, increasing either the sealing temperature or duration of seal, gives a

stronger, more complete seal. However over-sealing on a regular basis is not recommended, as

applying more heat can cause excessive damage to the plate being sealed. This in turn, would

reduce the number of times a particular plate can be resealed. Therefore a balance has to be

achieved, that gives an acceptable seal with the minimal plate damage or distortion.

Another optimisation factor to be taken into account, is the surface area of the plate.

A plate with thin raised rims around each well, will have a reduced surface area, compared to a

plate with wide raised rims. This would mean that less heat is needed to seal the thin rim plate in

comparison to the wide rim.

The pressure that the heater plate exerts during sealing, is pre-set and cannot be adjusted.

Plates that do not have any raised rims are not usually suitable for heat-sealing.

Temperature and Time Setting

The following table gives a guide line as to the sealing time and temperature for

different materials.

Plate Material

Polystyrene

Polypropylene

Polyethylene

For Sealing PCR Plates- No Skirt, Half Skirt

An adaptor will be required to seal plates which have well bottoms protruding past the plate skirt.

This is available from Porvair Limited.

Commands, Indications and Warnings.

The MiniSeal 2 System is software controlled, employing micro processor control.

All commands, indications and warnings are effected via the control screen.

Temperature Setting (° C)

145 to 165

150 to 175

150 to 170

MiniSeal 2 Issue 1

Time Setting (Seconds)

2 to 5