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3D Mosaic Imaging

Mosaic imaging is performed using a high-magnification objective to acquire continuous 3D (XYZ) images

of adjacent fields of view using the motorized stage, utilizing proprietary software to assemble the

images. The entire process from image acquisition to tiling can be fully automated.

Mosaic Imaging for 3D XYZ Construction

Composite images are quickly and easily prepared using the stitching function, to form an image over a wide area. 3D construction can

also be performed by acquiring images in the X, Y and Z directions. Tiled images can be enlarged in sections without losing resolution.

Automated from 3D Image Acquisition to Mosaic Imaging

Multi-area time-lapse software automates the process

from 3D image acquisition (using the motorized XY

stage) to stitching. The software can be used to easily

provides a view of the entire image acquired during

the mosaic imaging process.

CNS markers in normal mice

Objective

Zoom

Image acquisition numbers (XY): 32 x 38, 48 slices for each image

Courtesy of: Dr. Mark Ellisman PhD, Hiroyuki Hakozaki, MS Mark Ellisman

National Center for Microscopy and Imaging Research (NCMIR),

University of California, San Diego

Coordinate Information

Application/ Molecular Interaction Analysis

: PLAPON60x

: 2x

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