- ページ 22
顕微鏡 Olympus Fluoview FV1000のPDF 概要をオンラインで閲覧またはダウンロードできます。Olympus Fluoview FV1000 28 ページ。 Confocal microscope
Olympus Fluoview FV1000 にも： ユーザーマニュアル (42 ページ), 簡単な説明 (7 ページ), 簡単な説明 (7 ページ), ユーザーマニュアル (9 ページ), マニュアライン (3 ページ)
ページを印刷する
Expandability to Support Diverse Application.
Application
Molecular interaction and
molecular concentration
analysis
1.5
1
130
0.5
125
Pixels
0
105
125
105
130
Pixels
Laser Light stimulation
Acquires images while rapidly switching SIM scanner system
the built-in laser between imaging and
laser light stimulation.
Features tornado scanning for high-
efficiency bleaching using laser light
stimulation.
Multi-dimensional
time-lapse imaging
P2
P3
P1
P4
P5
3D mosaic imaging
TIRFM
FRET
Provides FRET analysis functions.
Diode laser offers exceptional stability
and long life.
Supports FRET efficiency
measurements using acceptor
photobleach method.
Multi-color imaging
Three-channel detector for
simultaneous acquisition of
fluorescence images from three
different dyes.
Sequential mode for acquisition of
fluorescence images without cross-talk.
Fluorescence can also be separated
using unmixing (only available on
spectral scan unit).
Colocalization analysis
Easily determine if labeled substances
are present locally in the same
locations.
Calculate of Pearson coefficients,
overlap coefficients and colocalization
indices.
* SIM scanner and TIRFM scanner cannot be installed on the same system.
** For more information about peripheral equipment, contact your Olympus dealer.
Standard Functions
Intracellular diffusion measurement
Calculation of diffusion coefficients for intracellular molecules, and analysis of
molecular binding and changes in molecular density.
—
Supports a wide range of methods (RICS/ccRICS, point FCS/point FCCS and
FRAP).
Software Required: Diffusion measurement package
Performs simultaneous imaging and laser light stimulation. Provides detailed
settings for laser light stimulation including position and timing.
Features tornado scanning for high-efficiency bleaching using laser light
stimulation.
Equipment Required: SIM scanner, laser combiner (dual fiber version)
Multi-point laser light stimulation system
Register multiple points for laser light stimulation, and program the respective
stimulation order, stimulation time and type of stimulation (continuous laser light
or pulse laser light).
Software Required: Multi-stimulation software
Long time-lapse system
Microscopes equipped with zero drift compensation (ZDC) acquire each image at
a set focus plane. The microscope CO
period for continuous imaging.
—
Equipment Required: IX81-ZDC microscope, CO
Multi-point scanning system
Register multiple points for repeated image acquisition. Efficiently observe multiple
cells in parallel on 35-mm dishes, microplates or chamber slides.
Software and Equipment Required:
Multi-area time-lapse software, motorized XY stage**
3D mosaic imaging system
Continuous imaging of adjacent fields of view and mosaic imaging to form a
—
composite image.
Acquisition of adjacent Z-series images for 3D mosaic imaging.
Software and Equipment Required:
Multipoint time-lapse software, motorized XY stage**
TIRFM imaging
Uses the laser from the laser combiner to provide evanescent illumination, for
imaging the movement of molecules near the glass surface, such as cell
—
membranes and adhesion factors.
Software and Equipment Required: TIRFM unit*, TIRF objective, high-
sensitivity CCD camera**, CCD camera control software**
CFP-YFP FRET
Ratio imaging and sensitized emission.
Available 440 nm diode laser is optimized for CFP-YFP FRET experiments
methods.
Diode laser offers exceptional stability and long life.
Equipment Required: LD 440 nm Laser
Imaging blue dyes
Available 405-nm laser for image acquisition of multi-stained samples labeled with
V-excitation fluorescent dyes such as DAPI, Hoechst and Alexa 405.
Equipment Required: LD 405 nm laser
Simultaneous four-color imaging
Fourth channel detector can be easily added to simultaneously acquire images of
four colors.
Equipment Required: 4-channel detector
High-accuracy colocalization analysis
New 60x oil-immersion objective offers image acquisition with exceptional
positional accuracy coefficient.
Equipment Required: PLAPON 60xOSC
Optional Functions
21
incubator maintains cell activity for a long
2
incubator
2