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Symptom

The contrast is low.

Chapter 8

Troubleshooting

2 Image Output

• Set the tone appropriately using the application software.

=> To make settings, see the instructions for the application software.

• Set the black level to the appropriate value using the application

software.

=> To make settings, see the instructions for the application software.

• Adjust the size of the field diaphragm so that it circumscribes the

viewfield.

• Adjust the size of the aperture diaphragm to approximately 70% to

80% of the numerical aperture of the objective.

• Use an appropriate filter.

When using a phase contrast microscope or an interference

microscope (two luminous fluxes or multiple luminous fluxes), insert

the specified filter. With a black and white image, contrast is improved

by inserting a green interference filter (GIF). Contrast in color images

is enhanced by inserting a filter having a complementary color to that

of the specimen.

• Adjust the ambient light around the microscope.

Make the room darker, or place caps on the binocular eyepiece, for

example.

• Check the contrast of the specimen itself.

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Corrective action