BD LSRFortessa 사용자 지침
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flowkon
Flow Cytometry User Instructions
at the University of Konstanz
FACSFortessa
The BD™ High Throughput Sampler (HTS) option for the BD LSRFortessa™ cell analyzer
provides rapid, fully automated sample acquisition from microtiter plates. In high-
throughput mode, the HTS can process a 96-well plate in fewer than 15 minutes.
Standard throughput mode can be selected for acquisition of larger sample volumes.
Preparing the HTS
Switch the acquisition control to plate mode.
This will switch off the DCM pump.
Unscrew the tube retainer (grey ring
DCM sleeve onto the SIT and carefully remove the sleeve.
Remove the DCM sleeve and replace it with the shorter SIT
protector.
Install the sample coupler on the SIT.
Check the level in the connected water bottle (> ~30 mL).
Switch on the HTS reader (on the backside).
Press the Run button (the HTS does not operate in STNDBY mode).
Initialisation and Priming the HTS
Note: Don't change PMTV of fluorescence channels after enabling compensation.
Operation of the loader is only possible via DIVA software – do not use the prime
button on the instrument.
When the BD DIVA software is already open, go HTS
Start BD DIVA software. The HTS will initialize automatically, otherwise go to HTS
Re-Initialize
Prime the HTS (Sheath tubing connected): HTS
Run your Experiment
See detailed instructions on HTS Programming.
– HTS User Instructions
TM
) that holds the
Re-Initialize.
Prime. 3x in total.