BD LSRFortessa 지침

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Job Aid

BD FACSCelesta™, BD LSRFortessa™, and

BD FACSymphony™ Analyzer

system using standard fluidics

This job aid contains instructions for performing a system flush using standard fluidics on BD LSRFortessa™, BD FACSCelesta™,

and BD FACSymphony™ Flow Cytometers. A system flush cleans the overall fluidics to remove debris and contaminants from the

sheath and waste tubing and flow cell. Perform the system flush at least every two weeks. See your instrument's users guide for

additional information.

Before you begin

You will need the following items:

• Stainless steel sheath tank

• 2 L of undiluted BD FACSClean™ Solution or

a freshly prepared 10% bleach solution

• 2 L of 1.5% BD

Detergent Solution

• 4 L of deionized (DI) water

Bypass the sheath filter

NOTE Do not run detergent, bleach, or ethanol through the

sheath filter. These fluids can break down the filter paper

within the filter body, causing particles to escape into the

sheath fluid, possibly clogging the flow cell.



Press the quick-disconnects on both sides of the filter

assembly.



Remove the filter assembly.



Connect the two fluid lines.



Empty the stainless steel sheath tank and rinse it with DI

water. Empty the waste tank, if needed.

families: Flushing the