Consort E3100 매뉴얼 - 페이지 10

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Running the gel

1. Mix the sample to be loaded with sample buffer (see solutions

2. Fill the unit with buffer until the gel is just flooded with buff-

3. Load the samples into the wells using pipettes. Multi-channel

4. Carefully place the lid on the tank and connect to a power

5. Typically gels are run at between 90 and 150 V. However,

All trays and the E3100 unit allow staining to be performed with-

out removing the gel from the tray if this is preferred.

1. Transfer the gel to a vessel containing the appropriate vol-

2. De-stain the gel for 10–30 minutes in distilled water again

3. Rinse the gel twice for a couple of seconds with distilled wa-

4. Transfer the gel to a UV Transilluminator.

5. The samples will often appear as brighter, clearer bands when

8 8

for common sample buffers). Usually 3 µl of sample buffer is

adequate but less may be used with sample volumes of less

than 10 µl.

er. This will give the fastest resolution times. For enhanced

quality of resolution of sample, fill the unit to 5 mm above

the gel.

pipettes can be used for loading samples with MultiPipette

compatible combs, see listing in accessories for identification

of these.

supply.

maximum voltage is indicated on the serial badge of each

unit. It should be noted that a higher voltage generally give

faster but poorer quality sample resolution.

ume of 0.5 µg/ml ethidium bromide stain for 15...30 minutes,

see solutions for stock stain concentration and adjust to the

volume used accordingly. The entire gel should be covered.

(Caution: ethidium bromide is a suspected carcinogen and the

necessary safety precautions should be undertaken).

ensuring the gel is completely immersed.

ter.

photographed or viewed using a gel documentation system.

However if the gel bands are too faint then the staining pro-

cedure should be adjusted so that there is less de-staining.

If there is too much background then the staining procedure

should be adjusted so that there is more de-staining.

E3xxx