Consort E3100 매뉴얼 - 페이지 8

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Gel pouring

Gel pouring with casting dams

Gel pouring

Gel pouring with casting dams

Gel pouring

with casting dams

1. Fit the casting dams over each end of the tray and place onto

2. Place the comb(s) in the grooves. Each tray has more than

3. Pour in the agarose carefully so as not to generate bubbles.

4. Allow the agarose to set, ensuring that the gel remains undis-

5. Carefully remove the gel casting gates and comb and transfer

Gel pouring with model E3100

Gel pouring

Gel pouring with model E3100

Gel pouring

with model E3100

1. Fit the casting gates into the grooves in the unit which are 10

2. Place the comb(s) in the grooves. Each tray has more than

3. Pour in the agarose carefully so as not to generate bubbles.

4. Allow the agarose to set, ensuring that the gel remains undis-

5. Carefully remove the gel casting gates and comb and transfer

6 6

a level surface. The dams should be fitted so that there is no

gap between the sides of the tray and the groove in the dams.

This will ensure that there is no possibility of gel leakage.

one comb grove so that multiple combs can be used. Using

multiple combs increases sample number available per gel

but decreases run length and care must be taken to ensure

that samples from the first wells do not migrate into the lanes

of the second comb wells.

Any bubbles that do occur can be smoothed to the edge of the

gel and dispersed using a pipette tip.

turbed.

the gel including tray to the main tank.

mm from the platinum wire. Ensure that these are seated as

deeply as possible. This will ensure that a good seal is formed

and that there is no possibility of gel leakage. A small amount

of vaseline or sealing grease on the bottom and side edges of

the gates will give further leakage protection.

one comb grove so that multiple combs can be used. Using

multiple combs increases sample number available per gel

but decreases run length and care must be taken to ensure

that samples from the first wells do not migrate into the lanes

of the second comb wells.

Any bubbles that do occur can be smoothed to the edge of the

gel and dispersed using a pipette tip.

turbed.

the gel including tray to the main tank.

E3xxx