Olympus CKX41 사용 설명서 - 페이지 12

{카테고리_이름} Olympus CKX41에 대한 사용 설명서을 온라인으로 검색하거나 PDF를 다운로드하세요. Olympus CKX41 36 페이지. Reflected fluorescence system
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USING THE CONTROLS

General Precautions for Observation

1. Confirm that the power supply unit has been set to the proper power supply voltage, power

supply frequency and burner type. Improper setting of these items may degrade burner service

life considerably.

2. Confirm that the power cord and connecting cords are connected properly.

3. When transmitted phase contrast or transmitted relief contrast observation is required, leave an open

position (BF) in the reflected fluorescent mirror slider. This position will allow the original colors to be

reproduced.

4. Stop down the field iris diaphragm until its image circumscribes the field of view. If it is decentered,

adjust centering using the Allen screwdriver. (P.16)

5. When using an LCPlanFl series objective, attach the correction cap, which compensates for the material

and thickness of the container used in observation. For the type of the correction cap to be used, refer

to the instruction manual for the CKX series culture microscopes.

6. The LUCPlanFLN20X/40X/60X, LCPlanFl40X and LUCPlanFl40X objectives with correction collar are

capable of compensating for the drop in resolution due to variance in the cover glass thickness. For

details, refer to the instruction manual for the CKX series culture microscopes.

7. When it is required to interrupt observation for a short period, use the shutter. (Repeated on-off of the

mercury burner will shorten its service life considerably.)

8. Precautions on the specimen color fading:

The system employs high-intensity excitation light to enable bright observation of dark fluorescent

specimens.

As a result, if high-power objectives are used frequently, color fading of the specimen occurs early,

degrading the view (contrast) of fluorescent images.

To delay color fading of the specimen and obtain favorable fluorescent images, it is recommended to

reduce the excitation light intensity a little.

The excitation light intensity can be reduced by using ND filters to an extent that does not hinder

observation. It is also effective to use the shutter frequently to avoid illuminating the specimen for a

longer period than required.

Color fading of the specimen can also be delayed using a commercially available color fading preventing

agent (DABCO, etc.).

The use of color fading preventing agent is recommended specially when you perform high-magnification

observation frequently.

# Note that color fading preventing agent cannot be used with certain specimens.