cytiva AKTA start 유지 관리 카드 - 페이지 8

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Note: We recommend to keep the instrument On to avoid

condensation in a cold room.

For more details on the procedure for turn on/off the UV

LED, refer to ÄKTA start Operating Instructions.

Note: Make sure that the UV flow cell is assembled tightly,

and the inlet and outlet tubes are fitted tightly to the UV

Monitor. No stray light should enter the UV Monitor.

Instruction

1. Immerse the buffer inlet tubing in DM water.

2. Flush the UV flow cell with DM water using

Pump. Make sure that there are no air bubbles in

the UV flow cell.

3. In the Settings and service screen, tap UV to

access the UV Monitor options.

4. In the UV screen, tap UV LED calibration.

5. Set the Light strength value to 500.

6. Tap Calibrate. A confirmation screen opens.

Select OK to automatically search the Light

strength value to get a minimum Signal

response of 2700 mV.

7. If the Signal value is above 2700 mV, tap Save.

If Signal is below 2700 mV use the arrows to

increase the Light strength until the Signal is

above 2700 mV.

Calibrate the Conductivity Monitor

Temperature sensor calibration

Instruction

1. Place a precision thermometer in the flow path

directly after the Conductivity cell and pump DM

water through system with a flow rate of 0.5 ml/

min.

2. Access Conductivity > Advanced calibration.

3. Note the temperature and type it into the Set

actual temp field, then tap Calibrate.

Sine generator calibration

Instruction

1. Use Manual run to empty the conductivity flow

path.

2. Disconnect the flow cell and dry it with a stream

of air.

3. Access Conductivity > Advanced calibration.

4. Tap Calibrate sine gen. The cell resistance

value should be 60 Mohms.

Conductivity flow cell calibration

Prerequisites

Calibration solution: 1.0 M NaCl or 100 mS/cm

conductivity standard solution.

Instruction

1. Fill the flow cell with conductivity standard

solution.

2. Access Conductivity > Calibration.

3. Note the current temperature of the calibration

solution in the conductivity flow cell as displayed

in the Cell temperature field.

4. In the Calibration screen, enter the conductivity

value at current temperature in the Set

conductivity field and then tap Calibrate.

Note:

For conductivity standard solutions, use the supplied

theoretical conductivity value at the current

temperature.

For 1.0 M NaCl calibration solution, read the conductivity

value at the current temperature from the graph

provided in the ÄKTA start Maintenance Manual.

Reference temperature setting

Instruction

1. Access Conductivity > Configuration.

2. Set the Set ref temp value to the temperature

shown in the Conductivity > Calibration

screen.

3. Tap Save.

Note:

The Cell constant value is set automatically when

calibration is performed. Do not change this value.

System performance test

System performance test is performed to make

sure the system is performing within acceptable

limits. It is recommended to run this method during

installation of the instrument or after replacement

of modules such as Pump, UV, Conductivity or

Valves. For a detailed description of the test, refer to

ÄKTA start Operating Instructions.

Required solutions

•

Buffer A: DM water

•

Buffer B: 1.0% Acetone, 1.0 M NaCl

•

Sample: 1.0% Acetone, 1.0 M NaCl (Buffer B)

ÄKTA start Maintenance Cue Card, 29024043 AF