Astrea Evolve AA20CRK Replacement Manual - Page 16

Browse online or download pdf Replacement Manual for Industrial Equipment Astrea Evolve AA20CRK. Astrea Evolve AA20CRK 19 pages. Wetted flow path for 200mm and 250mm chromatography columns

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Preparing for Column Use

After replacing the wetted flow path it is recommended before use these instruction are followed.

Hydrostatic Leak Test

The purpose of this test is to check that there are no leaks from the column and the column is ready

to use. Due to the materials of fabrication, when the column is initially exposed to pressure the

column will flex. It is necessary to perform the hydrostatic leak test using a two step procedure.

1. Ensure fixed end is correctly located and fixings tightened to the correct torque

setting. See table below.

Column Diameter

200mm

250mm

2. Fill the column with WFI or high quality water and position the adjuster at the

operating bed height.

3. Ensure the adjuster seal is wetted with buffer or other suitable liquid prior to

assembly. This helps 'lubricate' the seal to ensure correct seating.

4. Activate the adjuster seal

5. Ensure all air is removed from the column and any connections.

6. Attach a calibrated pressure gauge to the top inlet of the column

7. Raise the column pressure until 3bar (45psi) and close bottom inlet valve. Wait

15mins for the pressure to stabilize.

8. Open the valve and increase the pressure to maximum working pressure 4bar (58psi).

9. Wait 15mins for the pressure to stabilise. Check the pressure and ensure pressure is 4

bar (58psi).

10. Wait for 15mins.

Due the nature of materials it is likely there will be a small pressure drop, however, this

should not exceed 0.1 bar.

Leveling the 200mm & 250mm Columns

200mm and 250mm columns are fitted with height adjustable wheels and a spirit level is

provided. This enables the column to be leveled or increase ground clearance.

For hygiene the adjustability mechanism is covered by a sliding sleeve.

Caution! Before attempting to adjust the height of the support leg, carefully slacken the

locknut located between the foot and the support leg.

Please refer to Figure 1.13.

1. Slide sleeve upwards to gain access to adjuster nuts.

2. Using the correct spanner/wrench and loosen the locking nut. Fit a spanner to the

Height adjust nut and rotate the stud to raise or lower the caster. There is approximately

36mm free vertical movement available in each foot, and continuing to turn the screw

beyond this point will detach the foot from the leg.

3. When the height has been adjusted, replace the sleeve.

Torque Setting

Ft lbf

22.1