Cole Parmer 1200 Series User Manual - Page 10

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Basic Operation

Simple OPERATION INSTRUCTIONS are printed on the front panel of your Cole

Parmer 1200/1201. Three Basic Operations: Sample Preparation and Analysis,

Concentration Mode-C, and Factor Mode are shown in this section.

Spectrophotometer Warm-up and 0%T Check

Turn on the spectrophotometer by turning on the Power Switch (IO). Allow 15

minutes for the instrument to warm up.

Even through the 0%T is pre-set, it may shift due to unexpected factors like

temperature change. To check 0%T, simply insert the Black Block (item

number: S-1200-111, used to check the 0%T) into the Cuvette Holder and close

the lid. Select T mode, the display should be 00.0%T. If not, press the 0%T

button to set 0%T.

NOTE: If you lose the Black Block, you may use any opaque object to block the light

beam to the detector to carry out 0%T Check.

Select either the T (Transmittance Mode) or A (Absorbance Mode) by pressing

the MODE button until the red light for T or A is on.

Sample Preparation

Select the desired wavelength by turning the WAVELENGTH control knob.

Make a blank reference solution by filling a clean square cuvette (or test tube)

half full with distilled/de-ionized water or other specified solvent. Wipe the cuvette

with tissue to remove the fingerprints or droplets of liquid.

Insert the blank cuvette into Sample Compartment and close the cover.

Set 0.000A or 100%T with the 0A/100%T (∧) button.

NOTE: This step fulfills the instruction on the front of the spectrophotometer (Set Full

Scale).

Remove the blank square cuvette or test tube. Set it aside in case that you may

need to adjust the 0A/100%T using ∧ button later (i.e. change the wavelength).

Sample Analysis

Rinse a second square cuvette with a small amount of the sample solution to be

tested. Fill the square cuvette half full and wipe it.

10.

Put the sample cuvette in the Sample Compartment. Close the cover.

11.

Read the T or A from the Digital Display window. Remove the sample cuvette or

test tube.

12.

If you are to test the same sample at other wavelengths, repeat step 4

each wavelength.

13.

For each new sample you analyze, repeat step 3

11.

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