AmScope B200 Operation Instruction Manual - Page 9

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of operation. Rotation clockwise makes tension decrease, and by contraries, rotation

counterclockwise makes tension increase.

4.7 Usage of immersion oil objective

The 100X objective of B series microscope can be used for observation even if

without immersion oil. However, adding moderate immersion oil between the front

lens of 100X objective and the cover glass of specimen can make the image clearer.

Please pay attention that air bubble and impurity can't be in the immersion oil,

otherwise, the image would be affected.

First of all, take 40X objective which has been focused well out from bright path,

then take 100X objective into bright path. At this time, nosepiece or stage moving

knob should be turned slightly, and also slightly turn fine focusing knob to clear away

the air bubble of immersion oil, otherwise, the air bubble would affect the image

badly.

▲After immersion oil used, the oil of specimen and the microscope surface should be

soon cleaned by absorbent cotton, lens paper, gauze or soft cotton cloth with

moderate mixture of pure industrial alcohol and ether (proportion 1:4)

▲Standard thickness 0.17mm cover glass should be chosen when high times objective

used, and thickness error should be within 0.01mm, otherwise, image definition

would be affected.

4.8 Mirror (Shown in Fig.4)

Mirror of B series microscope is optional, and it is used when electrical power isn't

supplied.

Mirror doesn't be suggested as illumination source when 100X objective used.