Active Motif 53080 Manual - Page 11

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Using the Cooled Sonication Platform

Benchtop Tube Coolers

The Benchtop Tube Coolers can keep the temperature of a sample at or below 4°C for up to

2 hours of sonication if the cooler has been placed in a -20°C freezer overnight. If a large amount

of time will pass between each sonication, return the cooler be to a -20°C freezer between

samples. Do not place coolers at -80°C for more than 30 minutes as they may be damaged.

Note:

If the Tube Cooler was kept in a -20°C freezer overnight, it will be sufficiently

cold to freeze your samples in about 5 minutes, unless you begin sonication.

Therefore, to prevent your sample from freezing, do not place it in the cooler

until you are ready to begin sonication. Keep your sample on ice and the cooler

in the freezer until all preparations for sonication have been completed.

Sonication

Ensure that the converter/probe is positioned properly in the converter/probe bracket and that

the platform settings are those that you determined to be appropriate for your tube type and

sample volume. If need be, reposition the converter/probe to the mark you placed on it and

re-zero the platform, then re-set it to its desired position.

Turn on your probe sonicator and adjust its settings to your preference. Press the latch and move

the platform down the rod. Place an open tube containing your sample in the Tube Cooler, then

place the cooler in the platform stand. Raise the platform until it is against the Stop Collar, and

ensure that the appropriate counter setting has been reached. Lift the tube up and move it around

slightly to verify that all is as expected. After verifying this, ensure that the tube is pressed down

all the way in the cooler. You are now ready to begin sonication following your protocol.

After sonication is complete, press the latch and lower the platform so that you can remove the

sample and place it on ice. Process additional samples, or clean the sonicator probe per your lab' s

standard practices, and return the Tube Cooler to the -20°C freezer.