Olympus BX60 Instructions Manual - Page 25

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Fig. 33

I Using the Aperture Iris Diaphragm

• Reflected light BrightfieM Observation

In general, a good image is obtained if the diaphragm is stopped down

to 7080% of tha objective's numerical aperture.

• Reflected light Oarkfield Observation

Always keep the aperture iris diaphragm knob @ pushed in to leave the

diaphragm open.

0 Depending on the specimen, an image with good contrast and little

Rare may sometimes be obtained by keeping the aperture iris diaphragm

stopped down a Unto more. Please experiment with this to see if it

works with the particular specimen.

1 Using the Pinhole Diaphragm

(Ras, 32.33) I

O When using a 2S0X objective, use the pinhole diaphragm to enhance

the effect of the aperture iris diaphragm.

1. Push in the aperture iris diaphragm knob ® to open the diaphragm.

2. Pull out the pinhole knob© to bring the pinhole diaphragm into the light

path. (Fig. 33)

* Tha pinhole diaphragm is placed at the same position as the

aperture iris diaphragm, and centration may bo lost when the

aperture iris diaphragm Is adjusted.

3. Place a mirror or other highly reflective specimen on the stage. With the

eyepieces in place, rotate the fine adjustment knob in the direction where

the specimen and objective move away from each ether unta the contour

of tha pmhoie becomes voible.

4. At this point if centration of the pinhole diaphragm is imprecise, use the

two aperture tris diaphragm centering knobs (3) to adjust the centration.

5. Bring the specimen into focus again.

* If the aperture iris diaphragm is stopped down when using the

pinhole diaphragm, flare may occur.

* The pinhole diaphragm is placed so that it can be centered at the

same position as the aperture iris diaphragm. However, due to

constfuction considerations, e certain play ftKTffra. This is required

for the pinhole diaphragm performance and it does not indicate

a malfunction.

* When using the pinhole diaphragm, contaminants on the eye

pieces and photo-eyepiece may become noticeable. To prevent

this, dean eyepieces periodically.

Using the Filters

(Fig. 34)

Engage the filters suitable for the particular observation.

Fig. 34