Olympus U-RFL-T Instructions Manual - Page 15

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Fig. 2

Centering the Aperture Iris Diaphragm (BX3-URA)

} This operation is not required with the BX3-RFAS code fluorescence

illuminator

1. Close the light path by sliding the shutter knob @ to position marked {.

2. Engage a mirror unit other than the ones for U-excitation (U-FUW, U-FUN,

U-FUNA) in the light path by rotating the turret.

3. Engage the 10X objective in the light path and lace the U-CST centering

target on the stage.

4. Open the light path by sliding the shutter knob to position marked \.

5. Move the white surface with cross lines of the U-CST until the cross lines

are overlaid on the center of field.

6. Turn the revolving nosepiece to engage the empty place (the objective

cap should be removed) in the light path.

7. Pull out the aperture iris diaphragm knob 2 to minimize the aperture iris

diameter.

8. Pull out the field iris diaphragm knob 3 to minimize the field iris

diaphragm.

Now the aperture iris image should be visible on the U-CST.

9. Fit the Allen screwdriver in the two aperture iris centering screws | and

adjust so that the bright dot of the aperture iris image comes on the

image center.

Effects of Aperture Iris Diaphragm

The aperture iris diagram helps adjust the brightness of the observed

image and improve the contrast.

} The aperture iris diaphragm cannot be used as a substitute to the shutter

even when it is stopped down to the minimum aperture.

(Fig. 2)