Nikon A1+ User Manual - Page 26

Browse online or download pdf User Manual for Microscope Nikon A1+. Nikon A1+ 28 pages. Lbic nikon confocal
Also for Nikon A1+: Standard Operating Procedure (15 pages)

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Perfect Focus System (PFS) Operation

PFS provides real time focus correction on a plane of interest during time lapse

experiments carried out above room temperature. PFS is required because the

microscope components expand as they heat which can cause the focus to drift. The

coverslip/medium interface is tracked and the distance from the interface to the plane of

focus is recorded as an offset value. If a change in the distance from the coverslip

interface is detected, the lens will move in the same direction to compensate, this

maintains the same offset value and therefore the focal plane is unchanged.

1. Focus on the sample

2. On the front of the microscope a green LED will

light next to the word "Focus" if the PFS can be

turned on. This indicates the refractive index

interface has been found.

3. Press the On button the front of the microscope

to enable PFS

4. Check the focus again now PFS has been

turned on, you may have to adjust it again

5. To change focus while PFS is enabled, use the PFS offset

controller wheel

6. The blue button on the side of the controller toggles between

course/fine focus