Nikon Eclipse Ti TIFT 1454 Manual

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Total Internal Reflection Fluorescence microscope

Nikon Eclipse Ti

Principal:

• Laser is pointed at an angle at specimen

• Due to this angle the laser will totally reflect

on glass/medium interface and creates an evanescent wave above the glass

• Thickness of wave is around 200nm, but decays exponentially

• Possible to only excite fluorophores in membrane, without background signal of the cytoplasm

Benefits:

• Extreme low background signal

• Perfect for membrane studies

• CCD Camera attached will provide in fast imaging

Contact

• Martijn de Gruiter

• Gert-Jan Kremers

Martijn de Gruiter

Source: NIKON

Be-343

tel. 31105

Be-346

tel. 43578

Manual: TIRF Microscope

Erasmus OIC

Ee-1454