Nikon Eclipse Ti TIFT 1454 Manual - Page 3

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Start system

• Turn on the power by the power socket (on the floor under the table)

• Switch the mercury lamp on and then ignite it.

o If not needed don't turn the mercury lamp on

o The unit on the shelf closest to the door

• Turn on all devices with a button on the shelf and the table.

o Camera and laser box are automatically enabled

• Next turn on the device with a key: Laser control panel

• Start the computer and start the program Metamorph.

o Select suited user account.

Live Cell imaging:

• When imaging at 37

• The heating device to maintain your cells uses water.

o fill reservoir with demineralized water up to level of CO2 tube

• Allow the system to warm up for 15-30 minutes

• Settings for the heating unit are paper on the wall, choose your settings

o General settings for cells on a coverslip:

o top heater 40.5 ° C

o bath heater 38.5 ° C

o stage heater 38.5 ° C

o lens heater 37 ° C

• Ensure that the cells are grown on a glass coverslip

• Place the slide in the metal ring, tighten firmly but not too hard because it will break the

glass

• Add 1-2 ml of the medium from the appropriate well. There is a convex surface

• Move with a paper towel around the edge of the coverslip from below with a little pressure,

if no medium leaks from the ring your seal is good.

• Wipe clean the underside of the slide with ethanol to prevent contamination of the

objective and microscope

Martijn de Gruiter

Manual: TIRF Microscope

Erasmus OIC

Ee-1454