Nikon Optiphot Quick Reference Manual - Page 3

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Fine Focus

Coarse Focus

Transmitted

Light

ND Filters

"Photo" switch

Brightfield lamp power

ND2

ND4

ND16

ND2+ND4

ND2+ND16

ND4+ND16

ND2+ND4+ND16

Table 2. Neutral density filters and combinations for transmitted light.

July 29, 2016

Digital Microscopy Center, University of Washington

Condenser focus

Figure 1. Sub-stage Controls

Filter

Light Reduction

1/2

1/4

1/16

1/8

1/32

1/64

1/128

Substage field diaphragm

Brightfield lamp voltage

% Transmission

50%

25%

6.25%

12.5%

3.125%

1.562%

0.781%