Olympus Fluoview FV1000 Korte instructies - Pagina 5
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Olympus FV1000
3. Switching on the microscope
1. Follow the procedure by the numbers:
1
,
2. Switch on the HBO lamp (no. 1, underneath the computer table).
3. Switch on buttons no. 2 and 3. Microscope stand, laser and scan controllers, and the
computer start automatically.
4. Start the Olympus software: double click on "FV1000 1.7.
5. Select your User ID, type in your password, and press OK.
6. Do not perform the next step until the software start up is completed. If you get any
error messages at this step consult the trouble shooting guide.
7. This microscope provides the following laser lines:
405 nm
440 nm
491 nm
532 nm
559 nm
655 nm
The lasers for 405, 440, and 655 nm are controlled entirely through the Olympus
software. These lasers are switched on by selecting an acquisition protocol from the
dye list in the "Image Acquisition" window or by directly clicking on the laser box in
the "Acquisition Setting" window.
The lasers for 491, 532, and 559 nm have to be switched on manually.
Note, the software will not recognize, if they are not switched on.
Switch on only the lasers you need.
8. To switch on 491 and 532 nm, turn the ignition key (no. 4) to the vertical position. The
LED "LASER ON" will turn green. LED "TEMP LOCK may flash for 60 sec. Then
all four LEDs should be green.
9. To switch on 559 nm (no. 5), wait until the LED 'TEMP' stops blinking. Then turn the
ignition key (no. 5) to the ON position. The red LEDs "Laser" will blink for a few
min. Then they should stay red.
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Start".
Short instructions