Olympus Fluoview-1000 Gebruikershandleiding - Pagina 4

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Olympus Fluoview-1000 Gebruikershandleiding

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1. Add a Scale Bar to Your File

V.M. Bloedel Hearing Research Center, Core for Communication Research

Center on Human Development and Disability, Digital Microscopy Center

FV-1000 Optical Specifications

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Start-up problems.

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Scans, but no image.

The confocal image appears unevenly illuminated:

The epifluorescent or tungsten images appear unevenly illuminated:

Figure 1. Main Fluoview window.

Figure 2. Enlarged view of Fluoview windows.

Figure 3. Microscope Controller Window

Figure 4. Image Acquisition Window - Basic controls for Finding the Image

Figure 5. Channel Settings for the Photomultiplier Tube (PMT)

Figure 6. The Scan Mode and Scan Size settings of the Acquisition Setting Window.

Figure 7. Selecting laser lines and setting laser power

Figure 8. Scan Controls for Zoom, Pan and Rotation

Figure 9. Sequential Imaging Control

Figure 9A. Sequential Imaging Control

Figure 10. Z-Series Control Window

Figure 11. Z-Series Control Window

Figure 12. DIC and Focus Controls, Lower Half of the Microscope

Figure 13. Condenser and DIC Controls

Figure 14. Tilting the condenser.

Figure 15. The Stimulus Setting window for photoactivation

Figure 16. Photobleaching an ROI.

Figure 17. The toolbar item for control over display color

Figure 18. Fluoview appearance in Darkroom Color

Table 1. Mirror Buttons

Table 2. Objective Lenses Available

Table 3. Scan Speed and Acquisition Time

Table 4. Optimal Z-Step Values

Table 5. Pixel Size (µm) versus Box Size and Magnification at Zoom = 1

Table 6. Dichroic Mirrors and Barrier Filters

Table 7. Laser Lines on the FV-1000

Table 8. Index of Refraction for Common Mounting Materials

May 11, 2011

Olympus Fluoview-1000 User's Guide

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