Nikon Live Cell 2 Handmatig - Pagina 12

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Z-stacks

Although not an ideal imaging mode for wide-field techniques due

to the presence of out-of-focus light, it is possible to perform Z-

stacks which may be used for 3D visualisation or for maximum

intensity projections. Select the Z-stack tab in the ND Acquisition

window (Fig. 28).

1. Press reset to remove any previously saved z-positions.

2. Three options are available for setting Z-stack limits: Top

and Bottom (mark the limits of the stack), Symmetric

(mark the centre of the stack) and Asymmetric (set the

number of slices above and below the centre).

3. For the top-bottom option, whilst live imaging, adjust the

focus to find the top of your sample (use the Z value as a

guide) and click Top. Focus back through the sample to do

the same for the bottom plane of the sample. Stop imaging

to avoid sample bleaching.

Nikon Imaging Centre @ King's College London

Nikon

King's College London

Imaging Centre

Fig. 27

Fig. 28