Nikon N-STORM Bedieningshandleiding - Pagina 28

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(2)

In the dialog box, configure the settings as follows.

[Minimum Height]:

Specify the minimum intensity of the bright

points, which was checked in the previous step.

[Maximum Height]: 20000

[CCD Baseline]: 100

[3D]: On

For 2D



[3D]: Off

Click [>>] to make the following detailed settings.

[Minimum Width (nm)]: 200

[Maximum Width (nm)]: 700

For 2D



[Maximum Width (nm)]: 400

[Initial Fit Width (nm)]: 300

[Max Axial Ratio]: 2.5

For 2D



[Max Axial Ratio]: 1.3

[Max Displacement (pix)]: 1

Setting Screening

The bright point images that are excluded by Screening

of Identification Settings are not displayed on any

channel.

Upon the completion of setting, click [OK].

(3)

Click

[Start STORM Analysis].

(4)

Select [Drift Correction], set [Periods] for [1], and

then click [Test].

Test analysis is performed for the currently

displayed period.

Chapter 2

STORM Microscopy Operation

5-(2)

5-(4)