Olympus FV1200 MPE Podręcznik użytkownika - Strona 8

Przeglądaj online lub pobierz pdf Podręcznik użytkownika dla Mikroskop Olympus FV1200 MPE. Olympus FV1200 MPE 12 stron.

Olympus FV1200 User guide

Sequential Scanning Mode

In many cases, the bleed-through between multicolor can't be eliminated by a spectral setting. It can be

avoided by sequentially scanning the sample with one laser and one detector at a time.

1. Check the Sequential box and it will bring up the sequential scan information window. Choose between

Line or Frame mode.

Line: by line-by-line, it scans one channel with only a specific laser line and detector on and then sequentially

scans the next channel with only a another laser line and detector on.

Frame: it scans sequentially frame-by-frame; it finishes scanning one channel first and then scans the next

channel.

If there is no bleed-through

between the emissions of some

fluorophores you are using, you

can group them together to scan

them simultaneously as a group. Simply click on the fluorophore name on the list and drag to the other group.

(Note: it will be good to set up the acquisition condition for each channel before using the Sequential mode. To

do so, first in simultaneous mode (unchecked Sequential box), set the acquisition condition by turning on only

the specific laser for the specific channel. Once you set the imaging condition for each colors, check the

Sequential box.)

2. Scan with Focus x2 or XY Repeat button

not, stop scanning, and set the Scan speed at a slower rate and click

3. Save the image.

to see to check if there is any bleed-through. If

button to acquire the image.

June 23, 2015