Olympus FV1200 MPE Podręcznik użytkownika - Strona 9

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Olympus FV1200 User guide

G. Z-Series (xyz) Image Acquisition

Use this mode to obtain optical section through the depth (z dimension) of your sample that can be used for

3D visualization. Before starting the following procedure, make adjustment for xy multicolor imaging

condition as described above.

1. Click

button to scan.

2. Use the arrowheads

remote controller to focus into different Z-axial planes (large

arrowhead buttons shift a full step size and small ones a half

step size that you define).

3. When you find an upper limit of your sample by moving the

objective up, click Start Set button. Bring the objective down

until you find lower limit and click End Set button.

4. Determine the Step Size and the number of Slices, which correlate with each other. It is recommended to

set the step size similar to the optical section thickness of the objective you are using, so that there is no

gap between the optical sections upon projection into 3D. The step size can be fixed by checking the box.

5. Click Stop

button. Adjust the Scan Speed if needed, click Depth button ("Z"

will be appear on the XY button to become XYZ), and then click XYZ button.

6. When acquisition is done, Append

Next/SeriesDone

Stop button. If you need add additional sections,

click Append Next button (enter the number of

sections you want to add into the Append box

) or click

SeriesDone

acquisition.

7. Save the image.

H. Time-Series (xyt) Image Acquisition

Use this mode for imaging time-lapse of live specimens. Before

starting time series imaging, make adjustment for xy imaging as usual.

1. Click Time button

2. In the TimeScan option, enter the interval time (in second)

between one acquisition start and the next acquisition start (for

example; to set up a 1 min interval, type 60 and press Enter key. If

you put 0 and enter (FreeRun), the interval will be the acquisition

time that is required for each frame).

buttons or the focus knob on the

button will appear over the

to finish the

. It becomes XY_t.

June 23, 2015