Nikon A1+ Podręcznik użytkownika - Strona 15

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Z Stack Acquisition

1. To acquire a 3D dataset you need to define a Z stack

acquisition

2. Enable only the Z Stack tick box within the ND

Acquisition window

3. Choose the Z device from the dropdown box either Ti or

MCL Nanodrive Piezo. The piezo can move between z

planes at a faster rate but it only has a total travel range

of 100µm

4. Press the Reset button to clear any pre-existing values

5. Select the Defined by Top-Bottom

button

6. Press Scan

7. Turn the focus knob towards you to move to the top of the sample

then press the Top button

8. Now move the focus away from you to move to the bottom of the

sample then press the Bottom button

9. Press Scan again to halt the scanning process

10. To set the optimal step size between slices press this button. The calculated size

determines what step is required to produce optimal sampling according to the

Nyquist-Shannon theorem

11. To acquire the Z stack press the Run Now button at the bottom of

the ND Acquisition window

12.

While the stack is being acquired

a progress window will appear. Pressing Abort will stop the acquisition and close

the z stack window. Pressing Finish will stop the z stack but the stack window will

remain open.