Nikon C1si Podręcznik użytkownika - Strona 2
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Drukuj stronęC1Si Turn-On/ShutDown Procedures
Turn On:
1. Turn on arc lamp (Nikon Intensilight on shelf above table). This should be left on for
at least 30 minutes at a run and turned off for at least 30 minutes prior to turning on
again, this prevents the arc from flickering and wearing out prematurely
2. Turn on the lasers that you will use: (you don't need to turn them all on)
a. 2x Diodes on the laser bench: turn key from 12 o'clock to 3 o'clock.
b. 1x Diode on top of the control box: turn key and then push 'laser on' (green
button).
c. Argon-Ion multi-line: Turn the key clockwise (do not turn off switch).
3. Turn on the remote focus accessory: switch is on left hand side of box.
4. Turn on the control box (Nikon D-Eclipse C1). Don't start software until 'ready' light
is on.
5. Make sure the Z-stepper motor slider (located back-right of microscope) is pushed
down for manual operation (or up if you are going to use the remote focus
accessory).
6. Choose and carefully load 1 or 2 objective lenses. Center the lever so that both
lens sockets are in the up position. Use both hands and gently thread it until it is
finger tight. Do not over tighten it or it will get stuck.
Number
Objective
1
10x/0.30 NA
W Plan Fluor
2
20x/0.75 NA
air CFI Plan
Apo
3
40x NIR/ 0.8
NA W
4
40x/1.30 NA
oil Plan
Fluor
5
60x/1.2 NA
W NIR Apo
6
60x/1.4 NA
oil Plan Apo
VC
Dipping objectives are designed to form an image without a cover slip, whereas
immersion lenses require a cover slip. For ideal imaging, always use #1.5 cover slips.
You can then switch between the two different objectives that you have loaded by
turning the lever on the front so that it is in the middle (this is very important because
if it is not in the middle the objectives can come crashing down and get damaged),
pressing the black button on the top right, and carefully moving the carriage forward
or backwards.
Available Objectives
Theoretical
Depth of
Resolution
field (um)
(nm)
1017
14.4
407
2.31
381
2.03
235
0.77
250
~0.7
218
0.66
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Suggested
Transmitted
step size
light
(um)
5.76
DIC N1/10x
0.924
DIC N2/20x
0.812
DIC N2/40x
III
0.308
DIC N2/40x
II
~0.4
DIC N2/60x I
0.264
DIC N2/60x I
Working
Comments
Distance
0.3 mm
Water
Dipping
0.75 mm
Air
3.5 mm
Water
Dipping
0.20 mm
Oil
Immersion
0.27 mm
Water
Immersion
0.21 mm
Oil
Immersion