Nikon C1si Manual do utilizador - Página 2

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C1Si Turn-On/ShutDown Procedures

Turn On:

1. Turn on arc lamp (Nikon Intensilight on shelf above table). This should be left on for

at least 30 minutes at a run and turned off for at least 30 minutes prior to turning on

again, this prevents the arc from flickering and wearing out prematurely

2. Turn on the lasers that you will use: (you don't need to turn them all on)

a. 2x Diodes on the laser bench: turn key from 12 o'clock to 3 o'clock.

b. 1x Diode on top of the control box: turn key and then push 'laser on' (green

button).

c. Argon-Ion multi-line: Turn the key clockwise (do not turn off switch).

3. Turn on the remote focus accessory: switch is on left hand side of box.

4. Turn on the control box (Nikon D-Eclipse C1). Don't start software until 'ready' light

is on.

5. Make sure the Z-stepper motor slider (located back-right of microscope) is pushed

down for manual operation (or up if you are going to use the remote focus

accessory).

6. Choose and carefully load 1 or 2 objective lenses. Center the lever so that both

lens sockets are in the up position. Use both hands and gently thread it until it is

finger tight. Do not over tighten it or it will get stuck.

Number

Objective

10x/0.30 NA

W Plan Fluor

20x/0.75 NA

air CFI Plan

Apo

40x NIR/ 0.8

NA W

40x/1.30 NA

oil Plan

Fluor

60x/1.2 NA

W NIR Apo

60x/1.4 NA

oil Plan Apo

Dipping objectives are designed to form an image without a cover slip, whereas

immersion lenses require a cover slip. For ideal imaging, always use #1.5 cover slips.

You can then switch between the two different objectives that you have loaded by

turning the lever on the front so that it is in the middle (this is very important because

if it is not in the middle the objectives can come crashing down and get damaged),

pressing the black button on the top right, and carefully moving the carriage forward

or backwards.

Available Objectives

Theoretical

Depth of

Resolution

field (um)

(nm)

1017

14.4

407

2.31

381

2.03

235

0.77

250

~0.7

218

0.66

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Suggested

Transmitted

step size

light

(um)

5.76

DIC N1/10x

0.924

DIC N2/20x

0.812

DIC N2/40x

III

0.308

DIC N2/40x

~0.4

DIC N2/60x I

0.264

DIC N2/60x I

Working

Comments

Distance

0.3 mm

Water

Dipping

0.75 mm

Air

3.5 mm

Water

Dipping

0.20 mm

Oil

Immersion

0.27 mm

Water

Immersion

0.21 mm

Oil

Immersion