Nikon Labophot Instrukcja obsługi - Strona 25

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Failures

Causes

)

Actions

• Low contrasT in

To increase contrast optically, select phase

specimen

contrast. darkfield, or differential

interference

methods.

• Specimens should be stained a rather dark color, if

possible.

• In color photography, depending upon the speci-

men, red-blue separation staining (Mallory or Azan

methods etc.) is preferable to red-violet combina-

tion staining (H-E staining).

• In black-and-white photography, for low contrast

specimens a film of finer grain and higher contrast

is more suited (such as minicopy film).

• For general specimens a film of wider latitude and

finer grain is preferable.

• Insufficient N.A.~.

Use a large N.A. objective.

resolving

of objective

• For the same magnification,

increase power of

objective rather than that of eyepiece to attain

higher resolution and sharpness, even though depth

of field is reduced.

Excessive magnifi-~.

500 - 1000 times N.A. are magnification

limits for

cation

resolving power.

• Extraneous light~.

Darken the surroundings or place the cap on the

flare

entering the ocular

ocular finder.

fi nder

• Stray Iight entering

-----+.

Take care not to expose microscope and specimen

to direct sunlight and other intense lights.

• Inadequate use of-----.

Select best filter combination.

graph

filter

obtained

• Film of another

••

Note that, when using a daylight film, remarkably

make or emulsion NO.

different spectral sensitivities will result depending

upon the type, make, etc.

• Even though of same make, according to emulsion

number, different color rendition will be obtained.

Wrong power

• • Take picture in every case at the specified voltage.

source voltage

(Refer to P. 16)

• Incorrect exposure---->.

By inadequate exposure time, color rendition will

time

not be true on account of "reciprocity

law failure"

Then, with the help of exposure time indicator,

adjust exposure time according to characteristics of

film by means of NO filters, or compensate for

such fai Iure by means of CC fi Iters.

( Refer to Kodak Data)

) • Especially, for making color prints, it is recom-

film development

mended to contact the development laboratory.